1. What is the Bioluminex method?
2. How does Bioluminex work?
BioluminexTM solves many traditional issues of testing for chemical related toxins in complex sample matrices such as food, beverage, waste water, etc.

BioluminexTM is protected by patents: EP 0 588 139 B1 & US 6,238,928 & JP 0 246 032-93 & 6,673,563 & 6,340,572 & 6,017,722 If you have any questions, you may contact us at:  Ph: 949-419-0288 Fx: 949-419-0294
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WHAT IS BIOLUMINEX
4. Thin-Layer Chromatography
5. Principle of Bioluminescence
6. Vibrio fischeri
7. Vibrio fischeri
8. Five Examples
Corn-Ochratoxin
Muli-Toxin
Infant Formula-Strychnine
Wine-Carbaryl
Cayenne

1. What is the Bioluminex method?

First, high performance thin-layer chromatography (HPTLC) is used to separate multi-component mixtures into discrete substance zones.  After compound separation the development solvent or mobile phase is evaporated and the HPTLC plate is coated with a bioluminescent solution of Vibrio fischeri using a simple dipping procedure.  Single toxic compounds are then selectively identified as dark zones (areas of inhibited bacteria) on a luminescent background (areas of viable bacteria).  Results occur within seconds and last until the plate dries, approximately 30 minutes.  Data can be documented using a cooled CCD camera with a dark box or x-ray film. Additionally data can be quantitated directly on the TLC plate using densitometry.